The Study of Bio-Fuel Generation and Characteristics Analysis from Jatropha, Karanja and Styrax Officinalis L Seed Cakes

  • Minal Deshmukh School of Petroleum Engineering, MIT World Peace University, Pune, India
  • A. B. Marathe rincipal, HVPM College of Engineering, Amravati, India
  • Dinesh Bhutada School of Chemical Engineering, MIT World Peace University, Pune, India
Keywords: Jatropha seeds, karanja seeds, Styrax officinalis L seeds, Bio-fuels, Bio-mass, De-oiled seed cakes, Saccharification, Fermentation, Reaction kinetics, Transesterification.


Because of their high oil content in the kernel and ability to reduce greenhouse gas emissions, non-edible Jatropha, Karanja, and Styrax officinalis L seeds are primarily used in biodiesel production. Outstanding demand for biodiesel, as well as a huge amount of waste, is growing year after year. As a result, the research analysis includes the hydrolysis, saccharification, and fermentation of non-edible de-oiled cakes derived from the aforementioned plants. The study research includes the generation of bio-ethanol from non-edible cakes, as well as the de-oiled seed kernel, which is incubated at 200∘C, 25∘C, 30∘C, 35∘C, with pH 4, 5, 6, 7, and a holding time of 24 hours to produce the solid fuel. The compound cake is then fermented for 72 hours at 28∘C with Saccharomyces cerevisiae with a PH range of 5 to 6, and the substrate is autoclaved for 5 minutes at 15 psi pressure. Furthermore, this research study performed centrifuge harvesting at 10,000 rpm for 10 minutes at 4∘C. The study’s findings demonstrate the time-related conversion of sugar (DNSA) and alcohol production (potassium dichromate) treatment, which outperforms the prior acid hydrolysis process.


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Author Biographies

Minal Deshmukh, School of Petroleum Engineering, MIT World Peace University, Pune, India

inal Deshmukh is working as Assistant Professor in the School of Petroleum Engineering MIT World Peace University, Pune since January 2010. She has also worked as Production Engineer in sugar Factory. She completed her PhD in 2016 and M Tech in Chemical Engineering from Pune University. She received the Bachelor in Chemical Engineering in 2005 from Marathwada University, Aurangabad, Maharashtra, India. She is currently working on a DST funded project by Government of India regarding Biofuel. Her PhD work centres on “Synthesis of Biofuel and Organic Manure from Non Edible Deoiled Cake for Environmental Sustainability. Recently she is awarded with a Process Patent by Government of India based on her PhD work.

A. B. Marathe, rincipal, HVPM College of Engineering, Amravati, India

A. B. Marathe currently working as Principal, HVPM Collage of Engineering, Amravati. He has completed B. Tech (Chemical Engineering, Nagpur University), M.Tech (Chemical Engineering, Indian Institute of Technology, Bombay), Ph.D. (Chemical Engineering, Nagpur University) and M.B.A. He has total 41 years of professional experience out of which 6 years in Research & Development in Industry and 35 years in academic institution as teaching faculty at various positions of Assistant Professor, Associate professor, Professor and Head of Institution. Renewal sources of materials like oils & fats, Renewal sources of energy like solar energy & bio fuels, value added products from waste, Appropriate technology are some of the areas of his studies & research. He has 04 nos. of Indian patents granted to his credit. He is fellow of Indian Institute of Engineers and member of Academic council of Sant Gadge Baba Amravati University, Amravati.

Dinesh Bhutada, School of Chemical Engineering, MIT World Peace University, Pune, India

Dinesh Bhutada completed his Bachelors and PhD in Chemical Engineering SGB Amravati University and Masters from Bharati Vidyapeeth University Pune. He is working as Associate Professor in Chemical Engineering at MIT World Peace University Pune. He is having 15 years of experience in teaching and research. He writes quite frequently for various national and international academic journals and presents research work in national and international level conferences. He is having 5 Indian Patents to his credit in area of Environmental Engineering. He has published more than 20 research papers in peer reviewed journals and conferences. He has written more than 50 articles in newspapers and magazines for spreading awareness about Environment, Career Opportunities, soft skill and employability skills.


D. Kumar, and K. K. Pant, ‘Production and characterization of biocrude and biochar obtained from non-edible de-oiled seed cakes hydrothermal conversion’, Journal of analytical and applied pyrolysis, vol. 115, pp. 77–86, 2015.

C. H. Biradar, K.A. Subramanian, and M. G. Dastidar, ‘Production and fuel quality up-gradation of pyrolytic bio-oil from Jatropha Curcas de-oiled seed cake’, Fuel, vol. 119, pp. 81–89, 2014.

R. Muktham, A. S. Ball, S. K. Bhargava, and S. Bankupalli, ‘Bioethanol production from non-edible de-oiled Pongamiapinnata seed residue-optimization of acid hydrolysis followed by fermentation, Industrial Crops and Products, vol. 94, pp. 490–497, 2016.

D. Barik, and S. Murugan, ‘Assessment of sustainable biogas production from de-oiled seed cake of Karanja-an organic industrial waste from biodiesel industries’, Fuel, 148, pp. 25–31, 2015.

A. B. Fadhil, A. I. Ahmed, and H. A. Salih, ‘Production of liquid fuels and activated carbons from fish waste’, Fuel, vol. 187, pp. 435–445, 2017.

R. Sindhu, E. Gnansounou, S. Rebello, P. Binod, S. Varjani, I. S. Thakur, R. B. Nair and A. Pandey, ‘Conversion of food and kitchen waste to value-added products’, Journal of environmental management, vol. 241, pp. 619–630, 2019.

J. Thiagarajan, P.K. Srividhyaand P. Balasubramanian, ‘Thermogravimetric and decomposition analysis of jatropha, castor and pongamiadeoiled seed cakes’, International journal of innovations in engineering and technology, vol. 7, no. 2, pp. 417–425, 2016.

P. Mishra, G. Balachandar and D. Das, ‘Improvement in biohythane production using organic solid waste and distillery effluent’, Waste Management, vol. 66, pp. 70–78, 2017.

R. S. Malani, H. Sardar, Y. Malviya, A. Goyal, and V. S. Moholkar, ‘Ultrasound-intensified biodiesel production from mixed non-edible oil feedstock using heterogeneous acid catalyst supported on rubber de-oiled cake’, Industrial & Engineering Chemistry Research, vol. 57, no. 44, pp. 14926–14938, 2018.

D. Sut, R. S. Chutia, N. Bordoloi, R. Nazari, and R. Kataki, ‘Complete utilization of non-edible oil seeds of Cascabelathevetia through a cascade of approaches for biofuel and by-products’, Bioresource technology, vol. 213, pp. 111–120, 2016.

R. Muktham, A. S. Ball, S. K. Bhargava, and S. Bankupalli, ‘Study of thermal behavior of deoiledkaranja seed cake biomass: thermogravimetric analysis and pyrolysis kinetics’, Energy Science & Engineering, vol. 4, no. 1, pp. 86–95, 2016.

L. Lin, F. Xu, X. Ge, and Y. Li, ‘Improving the sustainability of organic waste management practices in the food-energy-water nexus: A comparative review of anaerobic digestion and composting’, Renewable and Sustainable Energy Reviews, vol. 89, pp. 151–167, 2018.

Y. Ma, Y. Yin, and Y. Liu, ‘A holistic approach for food waste management towards zero-solid disposal and energy/resource recovery, Bioresource technology, vol. 228, pp. 56–61, 2017.

M. Gohain, P. Bardhan, K. Laskar, S. Sarmah, M. Mandal, U. Bora, M. C. Kalita, V. V. Goud, and D. Deka, ‘Rhodotorulamucilaginosa: A source of heterogeneous catalyst for biodiesel production from yeast single cell oil and waste cooking oil’, Renewable Energy, vol. 160, pp. 220–230, 2020.

M. Carmona-Cabello, I. L. Garcia, D. Leiva-Candia, and M. P. Dorado, ‘Valorization of food waste based on its composition through the concept of biorefinery’, Current Opinion in Green and Sustainable Chemistry, vol. 14, pp. 67–79, 2018.

G. Russo, G. A. Vivaldi, B. De Gennaro and S. Camposeo, ‘Environmental sustainability of different soil management techniques in a high-density olive orchard’, Journal of Cleaner Production, vol. 107, pp. 498–508, 2015.

D. Kumar, and K. K. Pant, ‘Biorefinery solid cake waste to biocrude via hydrothermal treatment: optimization of process parameters using statistical approach’, Biomass Conversion and Biorefinery, vol. 6, no. 1, pp. 79–90, 2016.

S. Rezania, B. Oryani, J. Park, B. Hashemi, K. K. Yadav, E. E. Kwon, J. Hur, and J. Cho, ‘Review on transesterification of non-edible sources for biodiesel production with a focus on economic aspects, fuel properties and by-product applications’, Energy Conversion and Management, vol. 201, p. 112155, 2019.

R. S. Singh, N. Kaur, and J. F. Kennedy, ‘Pullulan production from agro-industrial waste and its applications in the food industry: A review, Carbohydrate polymers, vol. 217, pp. 46–57, 2019.

B. Chidambaranathan, S. Gopinath, R. Aravindraj, A. Devaraj, S. G. Krishnan, and J.K.S. Jeevaananthan, ‘The production of biodiesel from castor oil as a potential feedstock and its usage in compression ignition Engine: A comprehensive review’, Materials Today: Proceedings, 2020.

S. Gogoi, R. Nazari, N. Bordoloi, N. Bhuyan, D. Sut, L. Gogoi, and R. Kataki, ‘Influence of Temperature on Quality and Yield of Pyrolytic Products of Biofuel Process Wastes’, In Energy Recovery Processes from Wastes, Springer, Singapore, pp. 129–142, 2020.

P. Baranitharan, ‘Exergy analysis of a diesel engine fuelled with Aegle marmelos de-oiled seed cake pyrolysis oil opus’, Environmental Progress & Sustainable Energy, e13426, 2020.

J. Ha, S. Shim, T. Lee, Y. J. Kang, W. J. Hwang, H. Jeong, K. Laosatit, J. Lee, S. K. Kim, D. Satyawan, and P. Lestari, ‘Genome sequence of Jatropha curcas L, a non-edible biodiesel plant, provides a resource to improve seed-related traits’, Plant biotechnology journal, vol. 17, no. 2, pp. 517–530, 2019.

V. B. Shet, A. M. Palan, S. U. Rao, C. Varun, U. Aishwarya, S. Raja, L. C. Goveas, C. V. Rao, and P. Ujwal, ‘Comparison of response surface methodology and artificial neural network to enhance the release of reducing sugars from non-edible seed cake by autoclave assisted HCl hydrolysis’, 3 Biotech, vol. 8, no. 2, p. 127, 2018.

J. Thiagarajan, P. K. Srividhya, and P. Balasubramanian, ‘Thermal kinetics and syngas production on co-gasification of deoiledjatropha seed cake residues with wood chips’, International Journal of Renewable Energy Research (IJRER), vol. 8, no. 2, pp. 1105–1111, 2018.

I. Ahmad, M. Kano, S. Hasebe, ‘Dimensions and Analysis of Uncertainty in Industrial Modeling Process’, J. Chem. Eng. Jpn., vol. 51, pp. 1–11, 2018.

R.A. Perez, ‘Uncertainty Analysis of Computational Fluid Dynamics via Polynomial Chaos’, Ph.D. Thesis, Virginia Tech, Blacksburg, VA, USA, 2008.

S. Oladyshkin, W. Nowak, ‘Data-driven uncertainty quantification using the arbitrary polynomial chaos expansion’, Reliab. Eng. Syst. Saf., vol. 106, pp. 179–190, 2012.

M.M. Rajabi, B. Ataie-Ashtiani, C.T. Simmons, ‘Polynomial chaos expansions for uncertainty propagation and moment independent sensitivity analysis of seawater intrusion simulations’, J. Hydrol. 2015, 520, 101–122.

B. Sudret, A. Der Kiureghian, ‘Stochastic Finite Element Methods and Reliability: A State-of-the-Art Report; Department of Civil and Environmental Engineering’, University of California Berkeley: Berkeley, CA, USA, 2000.