Probing the Amyloid Peptide-Membrane Interaction Using a Liposome Model System

Authors

  • Liguo Tao School of Food and Biological Engineering, Jiangsu University, China
  • Yunpeng Cao Institute for Advanced Materials, Jiangsu University, China
  • Cuixia Ma School of Food and Biological Engineering, Jiangsu University, China
  • J. Wang Institute for Advanced Materials, Jiangsu University, China
  • L. Lin School of Food and Biological Engineering, Jiangsu University, China
  • L. Liu Institute for Advanced Materials, Jiangsu University, China
  • Mingdong Dong Interdisciplinary Nanoscience Center (iNANO), Aarhus University, 8000 Aarhus C, Denmark

DOI:

https://doi.org/10.13052/jsame2245-4551.2016001

Abstract

The aggregation of amyloid peptides is closely related to the pathogenesis
of degenerative diseases. More and more evidence implies that the proto
fibrillar intermediates rather than the mature amyloid fibrils are the toxic
species related to the membrane disruption. In this work we found that the
self-assembling intermediates of Aβ33-42 during early aggregation are able
to break down the liposome. During the process of amyloid peptide (Aβ33-42)
intermediate binding to liposome, the β-sheet secondary structure of peptide
took change on the molecular level which was characterized by circular
dichroism (CD) spectra. The small micelles were formed due to the disruption
of amyloid peptide, and further to grow into big irregular complexes with
further incubation, which is characterized by the assay of disrupting liposome
membrane and atomic force microscopy (AFM). This founding paved the way
to understand the interactions between the amyloid peptide and membranes,
and support the amyloid peptide nanostructure formed in the early stage of
aggregation has good affinity with membrane.

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Author Biographies

Liguo Tao, School of Food and Biological Engineering, Jiangsu University, China

L. Tao obtained the bachelor degree in biological sciences at Anhui Normal
University (Anhui, China). Liguo Tao is now a mater student of School of
Food and Biological Engineering in Jiangsu University (JiangSu, China),
The research interest focuses on the mechanism and antimicrobial activity
of antimicrobial peptides.

Yunpeng Cao, Institute for Advanced Materials, Jiangsu University, China

Y. Cao obtained the bachelor degree in Huaiyin Institute of Technology
(JiangSu, China). Yunpeng Cao is a master student of School of Materials
Science and Engineering in JiangSu University (JiangSu, China), and the
research project is studying on the interaction between amyloid peptide and
the liposome.

Cuixia Ma, School of Food and Biological Engineering, Jiangsu University, China

C. Ma was born in 1990, and she graduate from Yantai University in 2014
majoring in Marine biological resources. At present, she is studying Master
of food quality and safety in Jiangsu University.

J. Wang, Institute for Advanced Materials, Jiangsu University, China

J. Wang graduated from Chinese Academy of Science. Since 2013, he became
the assistant research in Institute forAdvanced Materials of Jiangsu University.
His work concentrates on the interaction of nanomaterials and proteins especially of disease-related biomarker, structure and function of complex.
Current research focuses on the interactions of nanomaterials and amyloid
protein.

L. Lin, School of Food and Biological Engineering, Jiangsu University, China

L. Lin was born in 1978. He received his Ph.D. degree from Jiangsu
University. His Ph.D. research was focused on Food Science. Since 2008,
he has been working at the Jiangsu University as associate research fellow
in the School of Food and Biological Engineering. he continue his postdoc
study at Interdisciplinary Nanoscience Center, Aarhus University (Denmark).
His research is now focused on antimicrobial preservation of natural products,
new food packaging materials, and the use of oils and fats active ingredients

L. Liu, Institute for Advanced Materials, Jiangsu University, China

L. Liu has completed his Ph.D in 2010 from National Center for Nanoscience
and Technology, China, after that he went to iNANO Center of Aarhus
University, Denmark for postdoctoral studies about 3 years working with
Prof. Flemming Besenbacher and Dr. Mingdong Dong. He became the Young
Distinguish Professor of Jiangsu University China, in 2013. Since 2014, he
has been the research and Jiangsu Specially-Appointed Professor of China.
His research interest focuses on the scanning probe microscopy, molecular
self-assembly of peptides and etc

Mingdong Dong, Interdisciplinary Nanoscience Center (iNANO), Aarhus University, 8000 Aarhus C, Denmark

M. Dong achieved Ph.D. in 2006 in Department of Physics and Astronomy,
then he continue his postdoc study at Rowland Institute, Harvard Harvard
University. Since 2009, he worked as an associate professor in Bio-SPM
group, Interdisciplinary Nanoscience Center, Aarhus University (Denmark).
Mingdong Dong long-term commits to the development and application of
scanning probe microscopy techniques. He successfully expanded biomolec-
ular imaging application in a liquid phase with high resolution. He developed
for the first time the use of force microscopy microsecond. His interests focus
on the structure of the material and research functions.

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Published

2023-03-18

How to Cite

Tao, L., Cao, Y., Ma, C., Wang, J., Lin, L., Liu, L., & Dong, M. (2023). Probing the Amyloid Peptide-Membrane Interaction Using a Liposome Model System. Journal of Self Assembly and Molecular Electronics, 4(1), 1–18. https://doi.org/10.13052/jsame2245-4551.2016001

Issue

2016: Vol 4 Iss 1 2016

Section

Articles